Pre-Owned Gene Targeting: A Practical Approach (Paperback) by Alexandra L Joyner
Alexandra L. Joyner

Pre-Owned Gene Targeting: A Practical Approach (Paperback) by Alexandra L Joyner

(No ratings yet)
Condition
Pre-Owned: Good
Seller Rating
4.6 out of 5 stars
13185 reviews

Key item features

  • ISBN: 9780199637928
  • Condition: Pre-Owned: Good
  • Trade paperback
  • 2nd ed.
  • Language: English
  • Pages: 312
  • Trade paperback (US). Glued binding. 312 p. Contains: Illustrations. Practical Approach, 212.
  • Since the publication of the first edition of Gene Targeting: A Practical Approach in 1993 there have been many advances in gene targeting and this new edition has been thoroughly updated and rewritten to include all the major new techniques. It provides not only tried-and-tested practical protocols but detailed guidance on their use and applications. As with the previous edition Gene Targeting: A Practical Approach 2e concentrates on gene targeting in mouse ES cells, but the techniques described can be easily adapted to applications in tissue culture including those for human cells. The first chapter covers the design of gene targeting vectors for mammalian cells and describes how to distinguish random integrations from homologous recombination. It is followed by a chapter on extending conventional gene targeting manipulations by using site-specific recombination using the Cre-loxP and Flp-FRT systems to produce 'clean' germline mutations and conditionally (in)activating genes. Chapter 3 describes methods for introducing DNA into ES cells for homologous recombination, selection and screening procedures for identifying and recovering targeted cell clones, and a simple method for establishing new ES cell lines. Chapter 4 discusses the pros and cons or aggregation versus blastocyst injection to create chimeras, focusing on the technical aspects of generating aggregation chimeras and then describes some of the uses of chimeras. The next topic covered is gene trap strategies; the structure, components, design, and modification of GT vectors, the various types of GT screens, and the molecular analysis of GT integrations. The final chapter explains the use of classical genetics in gene targeting and phenotype interpretation to create mutations and elucidate gene functions. Gene Targeting: A Practical Approach 2e will therefore be of great value to all researchers studying gene function.
Current price is $12.88
Price when purchased online
  • Free shipping
  • Free 30-day returns

How do you want your item?

How do you want your item?
Ships to
Arrives between Aug 27 - Aug 29
|
Sold and shipped by Alibris Books
4.574364808494502 out of 5 stars
4.574364808494502 stars out of 5, based on 13185 seller reviews(4.6)
Free 30-day returns

About this item

Product details

What is Pre-Owned: Good?

Has been read and shows minimal signs of wear like an owner's inscription or foxing, but no tearing and all pages are present.

What is the Walmart Pre-Owned Program?

Walmart Pre-Owned allows you to find previously owned, well-cared-for items from Walmart’s trusted & performance-managed sellers. Shopping Pre-Owned allows you to bring home the best-quality picks at even lower prices, in addition to extending the life of an item & reducing waste. Find your favorites & shop a range of conditions in every category.

See here for additional details & specific condition qualifications.

Why Walmart Pre-Owned?

Trusted sellers & quality items

Each Pre-Owned item listed comes from Walmart’s trusted performance-managed sellers, to ensure you get quality items.

Quality you can afford

Save even more on top brands & your most-loved items.

30-day free returns

Don’t love it? Most items offer a 30-day* free return policy, for added peace of mind.

Sustainability

Shopping Pre-Owned helps in extending the life of an item & reducing waste.

Product image for illustration purposes only. The item you receive may vary from the image in minor ways, such as slight differences in appearance, color, and/or design. *Exceptions apply during holiday season, and on certain electronics, collectibles, and jewelry.

Pre-Owned: Good

A well-cared-for item that has seen limited use but remains in great condition. Item and instructions are complete and included.

What is the Walmart Pre-Owned Program?

Walmart Pre-Owned allows you to find previously owned, well-cared-for items from Walmart’s trusted & performance-managed sellers. Shopping Pre-Owned allows you to bring home the best-quality picks at even lower prices, in addition to extending the life of an item & reducing waste. Find your favorites & shop a range of conditions in every category.

See here for additional details & specific condition qualifications.

Why Walmart Pre-Owned?

Trusted sellers & quality items

Each Pre-Owned item listed comes from Walmart’s trusted performance-managed sellers, to ensure you get quality items.

Quality you can afford

Save even more on top brands & your most-loved items.

30-day free returns

Don’t love it? Most items offer a 30-day* free return policy, for added peace of mind.

Sustainability

Shopping Pre-Owned helps in extending the life of an item & reducing waste.

Product image for illustration purposes only. The item you receive may vary from the image in minor ways, such as slight differences in appearance, color, and/or design. *Exceptions apply during holiday season, and on certain electronics, collectibles, and jewelry.

Specifications

Warranty

Warnings

Customer ratings & reviews

 

Resold at Walmart

0 out of 5 stars
0 ratings|0 reviews
This item does not have any reviews yet