Methods in Molecular Biology Base Editors: Methods and Protocols, Book 2606, (Hardcover)

Part I: Overview of BEs, Diverse Variants of BEs, and Computational Tools for Bes

1. Introduction and Perspectives of DNA Base Editors

Beomjong Song and Sangsu Bae

2. Web-Based Computational Tools for Base Editors

Gue-Ho Hwang and Sangsu Bae

Jinman Park and Hui Kwon Kim

4. Profiling Genome-Wide Specificity of dCpf1 Cytidine Base Editors Using Digenome-Seq

Daesik Kim

Part II: Application of BEs for Disease Modeling

5. Base Editing of Human Hematopoietic Stem Cells

Jing Zeng, Gabriele Casirati, My Anh Nguyen, Pietro Genovese, and Daniel E. Bauer

6. A/C Simultaneous Conversion Using the Dual Base Editor in Human Cells

Xiaohui Zhang, Yuting Guan, and Dali Li

7. Functional Analysis of Variants in BRCA1 Using CRISPR Base-Editors

Ji-Eun See and Yongsub Kim

8. Use of the Representative Base Editing Tool Target-AID to Introduce Pathogenic Mutations into Mice

Hiroki Sasaguri

9. Targeted Mutagenesis in Mice Using a Base Editor

Tae Yeong Jeong, Soo-Yeon Lim, Je Kyung Seong, and Kyoungmi Kim

Part III: Therapeutic Base Editing Strategies

10. Heterologous Expression and Purification of a CRISPR-Cas9-Based Adenine Base Editor

Seu-Na Lee, Hong-Su Jang, and Jae-Sung Woo

11. Delivering Base Editors In Vivo by Adeno-Associated Virus Vectors

Colin K.W. Lim, Angelo J. Miskalis, Pablo Perez-Pinera, and Thomas Gaj

12. The Delivery of ABE mRNA to the Adult Murine Liver by Lipid Nanoparticles (LNPs)

Dong Yang and Chun-Qing Song

13. Ex Vivo Base Editing Therapy with Chemically-Derived Hepatic Progenitors

Yohan Kim, Jaemin Jeong, and Dongho Choi

14. Application of Ba