The Role of Wnt Signalling in the Development of Somites and Neural Crest

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The Role of Wnt Signalling in the Development of Somites and Neural Crest

Format:  Paperback,

64 pages

Publisher: Springer Verlag

Publish Date: Jul 2008

ISBN-13: 9783540777267

ISBN-10: 3540777261

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The Wnt family of secreted signalling molecules controls a wide range of developmental processes in all metazoans. In this investigation, the authors have concentrated on the role that members of this family play during the development of the somites and the neural crest. Finally they have isolated a novel component of the Wnt signalling pathway called Naked Cuticle and investigated the role that this protein may play in both the previously mentioned processes.

Specifications

Publisher: Springer Verlag
Publish Date: Jul 2008
ISBN-13: 9783540777267
ISBN-10: 3540777261
Format: Paperback
Number of Pages: 64
Shipping Weight (in pounds): 0.4
Product in Inches (L x W x H): 6.0 x 9.0 x 0.25
Walmart No.: 9783540777267

Chapter outline

Introductionp. 1
Wnts and Developmentp. 1
Somite Formationp. 6
Induction, Delamination and Migration of Neural Crestp. 9
Induction of the Neural Crestp. 10
Delamination and Migration of the Neural Crestp. 12
Outline of Research Planp. 14
Wnt6 and Somitogenesisp. 14
The Regulation of cNkd1 Expressionp. 14
Wnt6 and Neural Crestp. 15
Materials and Methodsp. 15
Embryosp. 15
Culture of Wnt-Expressing Cellsp. 15
Operationsp. 16
BrdU Experimentp. 17
Neural Crest Cell Culturep. 17
HNK Staining Neural Crest Culturep. 17
Wnt6 siRNA RCAS Constructp. 17
Immunohistochemistry on Sectionsp. 18
Immunohistochemistry on Whole Embryosp. 18
Whole-Mount In Situ Hybridisationp. 19
Chemicalsp. 19
Anti-sense RNA Probesp. 19
DNA Linearisationp. 20
DNA Purificationp. 20
Probe Synthesisp. 21
Resultsp. 21
Wnt6 and Somitogenesisp. 21
Consequences of Ectoderm Removal on the Epithelialisation of Segmental Platep. 21
Effect of Wnt6 on the Development of Somitesp. 23
Wnt6 Rescues the Effect of Ectoderm Removalp. 23
Wnt6 Rescues the Effect of Medial Barrier Insertionp. 25
Effect of Wnt6 Expression on Muscle Developmentp. 28
Effect of Sfrp2 on Somite Developmentp. 29
The Regulation of cNkd1 Expressionp. 29
Normal Expression of cNkd1 in Chicken Embryos from HH8-HH20p. 29
Regulation of cNkd1 Expressionp. 31
Regulation of cNkd1 by Members of the Wnt Familyp. 33
Wnt6 and Neural Crestp. 36
Wnt6 Induces Neural Crest Production and Wnt1 Inhibits Neural Crest Inductionp. 36
Wnt6 Induces Neural Crest Production Through the Non-canonical Signalling Pathwayp. 37
Activation of the Non-canonical Pathway Through Dsh Induces Neural Crestp. 37
The Canonical Wnt Pathway Inhibits Neural Crest Inductionp. 39
Specific Inhibition of Wnt6 Reduces Neural Crest Productionp. 41
Discussionp. 43
Wnt6 and Somitogenesisp. 43
The Regulation of cNkd1 Expressionp. 47
Wnts and Neural Crest Inductionp. 49
Referencesp. 53

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